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Updated: May 8, 2026

Absolute Quantum Yield Measurement of Powder Samples
Published on: May 12, 2012
Eclipsing thermal lens spectroscopy for fluorescence quantum yield measurement
C Estupiñán-López1, C Tolentino Dominguez, R E de Araujo
1Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Cidade Universitária, Recife-PE, 50740-530, Brazil.
Abstract:
A modified spatial filtering method that improves the sensitivity of single-beam and mode-mismatched thermal lens spectroscopy (TLS) for fluorescence quantum yield measurement is presented. The method is based on the detection of the external part of a laser beam transmitted by the fluorescent sample (eclipsing detection mode). The experimental results show that the signal/noise (S/N) ratio of the absolute quantum yield of Rh6G can be enhanced up to ~1400% using the eclipsing detection mode on the TLS experimental setup. The method was evaluated by measuring the fluorescence quantum yield of varying concentration of ethanolic solutions of Rhodamine 6G.
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