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Updated: May 8, 2026

Absolute Quantum Yield Measurement of Powder Samples

Absolute Quantum Yield Measurement of Powder Samples

Published on: May 12, 2012

Eclipsing thermal lens spectroscopy for fluorescence quantum yield measurement.

C Estupiñán-López¹, C Tolentino Dominguez, R E de Araujo

¹Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Cidade Universitária, Recife-PE, 50740-530, Brazil.

|August 14, 2013

Summary

A new eclipsing detection mode enhances thermal lens spectroscopy (TLS) sensitivity for measuring fluorescence quantum yield. This method significantly improves the signal-to-noise ratio for accurate quantum yield determination.

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Area of Science:

Analytical Chemistry
Spectroscopy
Photochemistry

Background:

Thermal Lens Spectroscopy (TLS) is a sensitive technique for measuring thermal properties and photothermal effects.
Accurate determination of fluorescence quantum yield is crucial for understanding material photophysics and developing new optical materials.
Mode-mismatched TLS setups can suffer from limited sensitivity, hindering precise quantum yield measurements.

Purpose of the Study:

To present a modified spatial filtering method to enhance the sensitivity of single-beam, mode-mismatched TLS.
To improve the measurement accuracy of fluorescence quantum yield using the developed technique.
To validate the method by measuring the quantum yield of Rhodamine 6G solutions.

Main Methods:

Implementation of an eclipsing detection mode within a TLS experimental setup.
Detection of the external part of the laser beam transmitted through the fluorescent sample.
Measurement of the absolute quantum yield of Rhodamine 6G (Rh6G) at varying concentrations in ethanol.

Main Results:

The eclipsing detection mode significantly improved the signal-to-noise (S/N) ratio.
An enhancement of the S/N ratio up to approximately 1400% was achieved for absolute quantum yield measurements.
The method demonstrated reliable performance across a range of Rhodamine 6G concentrations.

Conclusions:

The modified spatial filtering method, utilizing eclipsing detection, substantially enhances TLS sensitivity for fluorescence quantum yield measurements.
This technique offers a significant improvement in signal-to-noise ratio, enabling more accurate determination of absolute quantum yields.
The developed method is effective for characterizing fluorescent samples, as demonstrated with Rhodamine 6G.