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Updated: May 8, 2026

Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
Published on: March 3, 2015
Mapping functional interaction sites of human prune C-terminal domain by NMR spectroscopy in human cell lysates
Donatella Diana1, Giovanni Smaldone, Pasquale De Antonellis
1Istituto di Biostrutture e Bioimmagini, CNR via Mezzocannone 16, 80134, Napoli (Italy).
Abstract:
Get well prune: The C-terminal third domain of h-prune is largely unfolded and involved in relevant protein-protein interactions, particularly with Nm23-H1 (see figure), GSK-3β and gelsolin. This study shows that protein functions mediated by protein-protein interactions can be accurately followed in cell lysates by using fast NMR spectroscopy, which could be easily used for a very efficient NMR drug-discovery strategy.
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