Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Microbes in Food Production01:29

Microbes in Food Production

Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated Multivariate Analysis and Desirability-Based Optimization of Milk-Whey Mixtures: Effects on Physicochemical Properties, Amino Acid Profile, and Nutritional Quality.

Foods (Basel, Switzerland)·2026
Same author

Stellate ganglion block using procaine in the treatment of herpes zoster ophthalmicus with keratitis after root canal treatment and post-herpetic pruritus in the third trimester of pregnancy and early lactation: a case report and review of the literature.

Frontiers in medicine·2026
Same author

Accelerating Proton Exchange in 1,8-Bis(dialkylamino)naphthalene Proton Sponges through Intramolecular Catalysis for CEST MRI.

Journal of the American Chemical Society·2026
Same author

Deuterated pH Probes Based on Ketoglutarate for Magnetic Resonance.

Journal of the American Chemical Society·2026
Same author

A one-health systematic review and meta-analysis (2020-2024) of global and regional prevalence of Mycobacterium bovis in dairy cattle and humans: Influence of diagnostic methods and herd-level determinants.

Preventive veterinary medicine·2026
Same author

Optimized cementitious immobilization of simulated cesium and barium radionuclides in borate waste solution by natural zeolite additives.

Environmental science and pollution research international·2026

Related Experiment Video

Updated: May 8, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Recent developments in manufacturing oligosaccharides with prebiotic functions.

Zoltán Kovács1, Eric Benjamins, Konrad Grau

  • 1Institute of Bioprocess Engineering and Pharmaceutical Technology, University of Applied Sciences Mittelhessen, Wiesenstrasse 14, 35390, Giessen, Germany, kovacs.zoltan@kmub.thm.de.

Advances in Biochemical Engineering/Biotechnology
|August 15, 2013
PubMed
Summary

Effective bioprocessing methods are crucial for meeting the growing demand for galactooligosaccharides (GOS) and fructooligosaccharides (FOS). Research focuses on improving manufacturing and purification techniques for these valuable prebiotics.

More Related Videos

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
08:38

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Published on: September 10, 2016

Related Experiment Videos

Last Updated: May 8, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods
08:38

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Published on: September 10, 2016

Area of Science:

  • Biotechnology
  • Food Science
  • Biochemistry

Background:

  • The global market for prebiotics like galactooligosaccharides (GOS) and fructooligosaccharides (FOS) is expanding.
  • GOS and FOS are recognized for their health benefits and desirable functional properties as food ingredients.
  • Current manufacturing processes for GOS and FOS face challenges related to incomplete conversion and product purification.

Purpose of the Study:

  • To review recent advancements in the bioprocessing and manufacturing of GOS and FOS.
  • To highlight similarities in the biosynthesis of lactose-based GOS and sucrose-based FOS.
  • To discuss bioengineering techniques for enhancing the purity of GOS and FOS.

Main Methods:

  • Utilizing whole cells or immobilized/free enzymes for oligosaccharide synthesis.
  • Investigating downstream separation technologies for carbohydrate enrichment.
  • Exploring additional enzymatic bioconversion and selective fermentation strategies.

Main Results:

  • Biosynthesis of GOS and FOS shares common reaction mechanisms and product formation pathways.
  • Biocatalysis yields a mixture of oligosaccharides, unreacted disaccharides, and monosaccharides.
  • Various bioengineering techniques show promise for purifying GOS and FOS.

Conclusions:

  • Optimized bioprocessing and purification strategies are essential for efficient GOS and FOS production.
  • Advances in bioengineering offer new avenues for manufacturing high-purity oligosaccharides.
  • Improved methods will support the increasing global demand for these functional food ingredients.