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Related Experiment Video

Updated: May 8, 2026

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
08:25

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Published on: September 26, 2022

Contact hypersensitivity models in mice.

Irving C Allen1

  • 1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 15, 2013
PubMed
Summary
This summary is machine-generated.

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This study details a protocol for inducing and assessing the contact hypersensitivity (CHS) reaction in mice. This model helps investigate cell-mediated immunity to epicutaneously applied allergens and their role in atopic diseases.

Area of Science:

  • Immunology
  • Dermatology

Background:

  • Contact hypersensitivity (CHS) reactions model cell-mediated immunity to epicutaneous allergens.
  • CHS involves distinct afferent (sensitization) and efferent (elicitation) phases.
  • While traditionally studied for Th1 responses, Th2 and Th17 cells also contribute to CHS.

Purpose of the Study:

  • To describe a standardized protocol for inducing and assessing the CHS reaction in mice.
  • To provide a reproducible method for studying immune responses to contact allergens.
  • To facilitate research into the mechanisms underlying CHS and its relevance to atopic diseases.

Main Methods:

  • Mice are sensitized epicutaneously with a contact allergen (hapten).
  • After a rest period, allergen is reapplied to an adjacent site (e.g., ear pinna) for elicitation.

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  • Immune responses are assessed at the elicitation site.

    • Main Results:

    • The protocol allows for reproducible induction and assessment of CHS in a murine model.
    • The CHS reaction can be evaluated using established immunological readouts.
    • This method supports the investigation of both Th1, Th2, and Th17 cell involvement.

    Conclusions:

    • The described protocol provides a robust method for studying CHS in mice.
    • Understanding CHS mechanisms is crucial for investigating atopic diseases in humans.
    • This standardized approach aids in advancing research on contact allergen-induced immune responses.