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Peptide Bonds

Peptide Bonds

A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Peptidoglycan Synthesis

Peptidoglycan Synthesis

Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan biosynthesis begins in...

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Updated: May 7, 2026

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Published on: November 2, 2011

Solid-phase peptide synthesis: an introduction.

Knud J Jensen¹

¹Department of Chemistry, Faculty of Sciences, University of Copenhagen, Frederiksberg, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|August 15, 2013

Summary

This summary is machine-generated.

This chapter introduces peptide chemistry, focusing on solid-phase peptide synthesis. It covers essential reagents and mechanisms, providing context for further study in the field.

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Area of Science:

Peptide Chemistry
Organic Chemistry
Biochemistry

Background:

Peptides are crucial biomolecules with diverse biological functions.
Understanding peptide chemistry is fundamental to various scientific disciplines.
Solid-phase peptide synthesis (SPPS) has revolutionized peptide production.

Purpose of the Study:

To provide a comprehensive introduction to peptide chemistry.
To offer an overview of solid-phase peptide synthesis (SPPS).
To present foundational knowledge on common reagents and reaction mechanisms in SPPS.

Main Methods:

Literature review of established peptide chemistry principles.
Detailed explanation of solid-phase peptide synthesis methodologies.
Discussion of commonly employed reagents and their roles in SPPS.

Main Results:

An overview of the fundamental principles of peptide chemistry.
A detailed look into the common reagents used in SPPS.
Explanation of key reaction mechanisms involved in SPPS.

Conclusions:

Solid-phase peptide synthesis is a versatile and widely used technique.
This chapter serves as a foundational guide to peptide chemistry and SPPS.
Readers are equipped with essential knowledge to understand subsequent chapters.