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Lectin-binding sites in clear cell acanthoma
M Akiyama1, K Hayakawa, Y Watanabe
1Department of Dermatology, Keio University School of Medicine, Tokyo, Japan.
Journal of Cutaneous Pathology
|August 1, 1990
Summary
Clear cell acanthoma (CCA) cells show lectin-binding sites similar to normal epidermis. This suggests clear cell acanthoma is a tumor originating from epidermal keratinocytes.
Area of Science:
- Dermatopathology
- Glycobiology
- Tumor Biology
Background:
- Clear cell acanthoma (CCA) is a benign skin tumor.
- Understanding the cellular origin of CCA is important for diagnosis and management.
- Lectin binding can identify cell surface and cytoplasmic carbohydrate structures.
Purpose of the Study:
- To investigate the lectin-binding sites in clear cell acanthoma.
- To compare lectin binding in CCA with normal epidermis.
- To determine the potential epidermal origin of CCA.
Main Methods:
- Used avidin-biotin complex (ABC) method with 9 different lectins.
- Examined formaldehyde-fixed, paraffin-embedded sections from 7 CCA lesions.
- Compared staining patterns with normal epidermal tissue.
Main Results:
- Positive staining on cell surfaces in CCA with Ricinus communis agglutinin I (RCA-I), Ricinus communis agglutinin II (RCA-II), and wheat germ agglutinin (WGA), similar to normal epidermis.
- Reduced reactivity with Concanavalin A (ConA) and peanut agglutinin (PNA) in CCA.
- Loss of staining with soybean agglutinin (SBA) and cytoplasmic staining with Bandeiraea simplicifolia agglutinin II (BSA-II) in CCA.
Conclusions:
- Lectin-binding profiles of proliferating cells in CCA resemble those of epidermal keratinocytes.
- The findings support the hypothesis that clear cell acanthoma originates from the epidermis.