Site-specific immobilization of biomolecules by a biocompatible reaction between terminal cysteine and
Ping Wang1, Chong-Jing Zhang, Ganchao Chen
1Department of Biology and Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, P. R. China. hongysun@cityu.edu.hk.
Abstract:
We report herein a new site-specific microarray immobilization method based on a biocompatible reaction between terminal cysteine and 2-cyanobenzothiazole (CBT). This immobilization strategy has been successfully applied to anchor small molecules, peptides and proteins onto microarrays.


