A biomimetic bitter receptor-based biosensor with high efficiency immobilization and purification using...

Area of Science:

• Biomimetic sensing
• Biosensor development
• Chemical sensing

Background:

• Mimicking biological sensing systems is crucial for advancing biosensor technology.
• Bitter receptors are key components in taste perception and have potential applications in biosensing.

Purpose of the Study:

• To develop a novel biomimetic bitter receptor-based biosensor for detecting specific bitter substances.
• To utilize bitter receptors as sensitive elements for the first time in a biosensor.
• To establish an effective immobilization strategy for bitter receptors.

Main Methods:

• A human bitter receptor (T2R4) was expressed and fused with a His6-tag.
• Membrane fractions containing T2R4 were immobilized on a quartz crystal microbalance (QCM) using an aptamer-based self-assembled strategy.
• The QCM device monitored T2R4 responses to bitter stimuli.

Main Results:

• The biosensor demonstrated high sensitivity and specificity for denatonium detection.
• Dose-dependent responses were observed within a specific concentration range.
• The aptamer-based method yielded a sensitivity of 1.21 kHz mM(-1), twice that of the SAM-based method.

Conclusions:

• The developed bitter receptor biosensor is effective for detecting denatonium and shows potential for broader bitter substance detection.
• The aptamer-based immobilization technique offers significant advantages for developing membrane receptor-based biosensors.
• This work advances bitter receptor immobilization and purification, benefiting future biosensor and molecular sensor array development.