[Qualitative analysis of accented CD158a receptor expression in NK-lymphocytes in women with reproductive failures]
Abstract:
Blood from 91 women that undergoing IVF cycle was analysed for NK cells CD158a expression using a monoclonal antibody and FACScan flow cytometer and CellQuest software (BD Bioscience, San Jose, USA). Patients were separated on 3 groups according implantation and pregnancy results in actual IVF cycle. 53 patients not became pregnant (IVF failure group F), 24 became pregnant after IVF with subsequent successful pregnancy (Pregnancy succes group PS) and 13 became pregnant with subsequently pregnancy failure (Pregnancy failure group PF). Average levels of of CD158a on NK cells were significant increase in patients that not became pregnant compared to pregnant group. However IVF failure patients have comparable average CD158a levels to reproductive success group. Patients with pregnancy failure have significant decreased CD158a levels compared to both IVF failure and reproduct success patients. A qualitative analysis of NK CD158a expression showed that 22/24 (92.8%) women who became pregnant and live birth had CD158a levels that were > 20 but < 65%. In contrast only 62.8% patients form IVF failure and 61.6% from Pregnancy failure group had CD158a expression on NK in this zones (corridor). 38.4% of patients from pregnancy failure group had CD158a expression levels lower than 20% and as a result significant decreased average value in whole group. In contrast IVF failure patients had increased CD158a expression in 9.5% cases and decreased in 27.7% and as a result similar average levels to pregnancy success groups. Decreased CD158a expression (< 20%) was significant predictive factor for reproductive failure (OR 10,7) Increased CD158a expression > 65%) was predictive factor for Implantation failure (OR 5,4; P = 0,09) Normal CD158a expression (> 20% but < 65%) was significant predictive for IVF implantation and Pregnancy success and as a result for common Reproduct success (OR 2,7; 6,87; 6,92). We found that normal NK CD158a expression is associated with successful IVF and pregnancy. Preference of qualitative analysis under simple average value comparison in case of bilateral distribution of parameters was shown.
