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Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

Overview

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Related Experiment Video

Updated: May 8, 2026

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
10:36

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

Published on: June 20, 2017

Assessing blood-brain barrier function using in vitro assays.

Joseph Bressler1, Katherine Clark, Cliona O'Driscoll

  • 1Hugo Moser Laboratory at the Kennedy Krieger, Kennedy Krieger Institute, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a method to isolate cow brain microvessels for blood-brain barrier (BBB) research. This technique aids in studying drug transport and BBB integrity, offering a cost-effective alternative to animal models.

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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

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Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
16:26

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

Related Experiment Videos

Last Updated: May 8, 2026

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
10:36

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

Published on: June 20, 2017

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
09:35

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

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Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
16:26

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

Area of Science:

  • Neuroscience
  • Pharmacology
  • Biotechnology

Background:

  • The blood-brain barrier (BBB) restricts chemical entry via tight junctions, low pinocytosis, and efflux transporters.
  • Lipophilic chemical permeability is limited by multidrug transporters, while polar nutrients utilize specific transporters.
  • Rodent models for BBB research are costly and have low throughput, necessitating alternative methods.

Purpose of the Study:

  • To describe a reproducible method for isolating large quantities of brain microvessels from bovine brains.
  • To outline techniques for assessing the purity and integrity of isolated microvessels.
  • To detail methods for evaluating BBB permeability and transport mechanisms using both microvessel and cell culture models.

Main Methods:

  • Isolation of brain microvessels using density gradient centrifugation and filtration.
  • Microscopic and enzymatic assessment of microvessel purity.
  • Permeability assays using fluorescein-labeled dextran uptake.
  • Evaluation of transendothelial electrical resistance and paracellular transport in cell culture models.

Main Results:

  • A protocol for isolating high-purity bovine brain microvessels was established.
  • The isolation method demonstrated a large dynamic range and low inter-day variability in permeability assays.
  • Established methods for evaluating BBB integrity and transport in both isolated microvessels and cell culture systems.

Conclusions:

  • The described method provides a scalable and cost-effective approach for obtaining brain microvessels for BBB research.
  • This technique facilitates the study of drug transport, nutrient uptake, and BBB integrity.
  • The combined use of isolated microvessels and cell culture models offers a comprehensive platform for BBB research.