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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Bioavailability Enhancement: Drug Permeability Enhancement01:27

Bioavailability Enhancement: Drug Permeability Enhancement

After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt secretion,...
Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients, maintaining...

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Related Experiment Video

Updated: May 8, 2026

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

Methods to assess tissue permeability.

Juan C Ibla1, Joseph Khoury

  • 1Department of Pediatrics, Genetics Medicine and Integrative Systems Biology, George Washington University, Washington, DC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 20, 2013
PubMed
Summary
This summary is machine-generated.

Understanding endothelial cell barrier function is crucial for organ health. This study presents methods to systematically measure organ permeability, aiding in the study of vascular leak and disease.

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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

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In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Related Experiment Videos

Last Updated: May 8, 2026

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
06:04

Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease

Published on: February 9, 2016

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
18:57

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers

Published on: October 17, 2013

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability
10:22

In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

Published on: October 19, 2018

Area of Science:

  • Physiology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Organ function relies on permeability barriers maintained by endothelial cells.
  • Disrupted endothelial barriers lead to vascular leak, implicated in disease and injury.
  • Understanding barrier mechanisms requires multidisciplinary approaches and in vivo data.

Purpose of the Study:

  • To describe simple, systematic methods for measuring organ permeability.
  • To provide tools for assessing endothelial barrier function in vivo.
  • To complement molecular findings with physiological data.

Main Methods:

  • Systematic measurement of organ permeability profiles.
  • Utilizing in vivo permeability data in mammalian models.
  • Employing a multidisciplinary approach to study barrier function.

Main Results:

  • Established methods for assessing organ permeability.
  • Demonstrated the utility of in vivo data for understanding barrier function.
  • Provided a framework for physiological validation of molecular findings.

Conclusions:

  • Accurate measurement of organ permeability is essential for understanding endothelial barrier function.
  • In vivo methods are critical for assessing physiological significance.
  • These methods support research into vascular leak and related diseases.