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Mechano-Node-Pore Sensing: A Rapid, Label-Free Platform for Multi-Parameter Single-Cell Viscoelastic Measurements
Published on: December 2, 2022
Generation and analysis of biosensors to measure mechanical forces within cells
Katharina Austen1, Carleen Kluger, Andrea Freikamp
1Group of Molecular Mechanotransduction, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
Abstract:
The inability to measure mechanical forces within cells has been limiting our understanding of how mechanical information is processed on the molecular level. In this chapter, we describe a method that allows the analysis of force propagation across distinct proteins within living cells using Förster resonance energy transfer (FRET)-based biosensors.
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