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Thermosensitive macroporous cryogels functionalized with bioactive chitosan/bemiparin nanoparticles.

Hazel Peniche1, Felisa Reyes-Ortega, María R Aguilar

  • 1Centro de Biomateriales, Universidad de La Habana, 10400, Havana, Cuba.

Macromolecular Bioscience
|August 20, 2013
PubMed
Summary

Novel thermosensitive poly(N-isopropylacrylamide) (polyNIPA) scaffolds with chitosan/bemiparin nanoparticles were created for tissue engineering. These macroporous cryogels offer controlled heparin release and show excellent biocompatibility.

Keywords:
bioactive bemiparin nanoparticlesimage-NMR microstructure characterizationmacroporous polymeric systemsthermosensitive cryogels

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Development of advanced materials for tissue engineering and drug delivery is crucial.
  • Thermosensitive polymers offer tunable properties for controlled release applications.
  • Heparin delivery systems require biocompatible and effective scaffolds.

Purpose of the Study:

  • To prepare and characterize thermosensitive macroporous scaffolds of poly(N-isopropylacrylamide) (polyNIPA) loaded with chitosan/bemiparin nanoparticles.
  • To evaluate the in vitro release kinetics and biological activity of bemiparin from the scaffolds.
  • To assess the cytocompatibility of the developed cryogels for tissue engineering applications.

Main Methods:

  • Free radical polymerization in cryogenic conditions to synthesize polyNIPA cryogels.
  • Complex coacervation for preparing chitosan/bemiparin nanoparticles.
  • Scanning Electron Microscopy (SEM) for structural analysis.
  • Differential Scanning Calorimetry (DSC) and image-NMR for material characterization.
  • In vitro release studies and cell proliferation assays (BaF32).

Main Results:

  • Chitosan/bemiparin nanoparticles (102 ± 6.5 nm) were successfully integrated into the macroporous polyNIPA cryogel structure.
  • Cryogel swelling behavior was dependent on nanoparticle concentration and temperature relative to the volume phase transition temperature (VPT).
  • Bemiparin release was modulated by chitosan presence, and released bemiparin retained biological activity.
  • The cryogels demonstrated no cytotoxicity to human fibroblast cells.

Conclusions:

  • Thermosensitive polyNIPA cryogels loaded with chitosan/bemiparin nanoparticles exhibit a highly porous structure and tunable swelling properties.
  • These scaffolds facilitate controlled release of biologically active bemiparin (heparin).
  • The developed cryogels are non-cytotoxic and suitable for tissue engineering and controlled heparin delivery.