Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 8, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

Microfluidic jet injection for delivering macromolecules into cells.

A Adamo1, O Roushdy, R Dokov

  • 1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Journal of Micromechanics and Microengineering : Structures, Devices, and Systems
|August 20, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasound assessment of diaphragmatic structure and function before and after orthotopic liver transplantation in MNGIE.

Journal of ultrasound·2026
Same author

Chromothripsis orchestrates leukemic transformation in blast phase MPN through targetable amplification of <i>DYRK1A</i>.

bioRxiv : the preprint server for biology·2023
Same author

Cell Squeeze: driving more effective CD8 T-cell activation through cytosolic antigen delivery.

Immuno-oncology technology·2022
Same author

Cost of illness of HER2-positive and metastatic and recurrent HER2-positive breast cancer - a Danish register-based study from 2005 to 2016.

BMC health services research·2022
Same author

Blood Vessel Detection Algorithm for Tissue Engineering and Quantitative Histology.

Annals of biomedical engineering·2022
Same author

Implementation of a quality management system according to ISO 9001:2015 standards in an Angiology Unit: an Italian experience.

Annali di igiene : medicina preventiva e di comunita·2022

A novel microfluidic injection system delivers macromolecules into single cells using a picoliter jet. This high-throughput method offers precise, quantitative intracellular delivery, improving upon existing techniques.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Current intracellular delivery methods face limitations in throughput and precision.
  • Efficient delivery of macromolecules into cells is crucial for various biological and therapeutic applications.

Purpose of the Study:

  • To introduce a microfluidic-based injection system for precise intracellular delivery.
  • To demonstrate the system's capability for quantitative and high-throughput macromolecule delivery.

Main Methods:

  • Development of a microfluidic system capable of generating a picoliter-jet.
  • Directing the picoliter-jet towards individual cells for targeted delivery.
  • Evaluation of system performance, design specifications, and delivery criteria.

More Related Videos

Picoinjection of Microfluidic Drops Without Metal Electrodes
09:20

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Related Experiment Videos

Last Updated: May 8, 2026

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

Picoinjection of Microfluidic Drops Without Metal Electrodes
09:20

Picoinjection of Microfluidic Drops Without Metal Electrodes

Published on: April 18, 2014

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
12:52

Micromanipulation Techniques Allowing Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators

Published on: May 12, 2018

Main Results:

  • Successful demonstration of intracellular delivery of macromolecules using the microfluidic system.
  • The system achieves precise, picoliter-scale delivery to individual cells.
  • The method shows potential for quantitative and high-throughput applications.

Conclusions:

  • The developed microfluidic injection system offers a significant advancement in intracellular delivery.
  • This technology overcomes key limitations of existing methods, enabling precise and efficient macromolecule delivery.
  • The system holds promise for applications in cell biology research and drug delivery.