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Updated: May 8, 2026
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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
Published on: February 28, 2017
Isolation of c-Kit+ human amniotic fluid stem cells from second trimester
Michela Pozzobon1, Martina Piccoli, Andrea Alex Schiavo
1Città della Speranza Foundation, Padova, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|August 21, 2013
Summary
Amniotic fluid-derived stem cells offer a promising, ethically sourced alternative for regenerative medicine. Researchers have developed a protocol to isolate c-Kit+ human amniotic fluid-derived stem cells from second-trimester amniocentesis samples.
Area of Science:
- Stem cell biology
- Regenerative medicine
- Cellular therapies
Background:
- Amniotic fluid-derived stem (AFS) cells are a fetal stem cell source.
- AFS cells avoid ethical concerns associated with embryonic stem cells.
- AFS cells bypass donor site morbidity from postnatal tissue biopsies.
Purpose of the Study:
- To establish a protocol for isolating specific AFS cell populations.
- To obtain c-Kit+ human AFS cells for potential therapeutic applications.
Main Methods:
- Utilized second-trimester amniocentesis samples.
- Developed and established a cell isolation and expansion protocol.
- Focused on identifying and isolating c-Kit+ AFS cells.
Main Results:
- Successfully established a protocol for AFS cell isolation.
- Demonstrated the feasibility of obtaining c-Kit+ human AFS cells.
- Confirmed the broad differentiation potential of AFS cells.
Conclusions:
- AFS cells represent an ethically viable and accessible stem cell source.
- The developed protocol enables the isolation of specific c-Kit+ AFS cells.
- These cells hold potential for future regenerative medicine strategies.
