Related Experiment Video
Updated: May 8, 2026
05:58
In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy
Published on: September 6, 2024
Label-free fluorimetric detection of histone using quaternized carbon dot-DNA nanobiohybrid
Subhabrata Maiti1, Krishnendu Das, Prasanta Kumar Das
1Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata - 700 032, India. bcpkd@iacs.res.in.
Chemical Communications (Cambridge, England)
|August 22, 2013
Abstract:
A fluorimetric histone sensing technique is developed using quaternized carbon dot-DNA nanobiohybrid. The method is simple, specific, and can detect a minimum of 0.2 ng mL(-1) histone.
Related Concept Videos
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...