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Updated: Mar 4, 2026

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
Published on: June 17, 2008
Protein migration via dissolution of ATP-stabilized condensate in a gradient of AMP
Shikha Shikha1, Sakshi Juneja1, Subhabrata Maiti1
1Department of Chemical Sciences, Indian Institute of Science Education and Research (IISER) Mohali, Knowledge City, Manauli-140306, India. smaiti@iisermohali.ac.in.
Abstract:
We report the condensate-forming tendency of bovine serum albumin (BSA) increases in the presence of adenosine triphosphate (ATP) in macromolecularly crowded media. However, in the presence of adenosine monophosphate (AMP), the condensate does not form, and the ATP-stabilized condensate dissolves. Furthermore, we report protein migration and spatial tuning of BSA-mediated proton transfer catalysis towards an AMP gradient via the dissolution of ATP-stabilized condensates (dialytaxis). This dissolution-based functional protein mobility may have implications in active biocolloidal studies.
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