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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
A workflow from untargeted LC-MS profiling to targeted natural product isolation
Damien L Callahan1, Candace E Elliott
1Metabolomics Australia, School of Botany, The University of Melbourne, Parkville, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2013
Summary
Liquid chromatography-mass spectrometry (LC-MS) detects diverse metabolites. This method uses reversed-phase C18 LC-MS for broad metabolite detection and targeted isolation.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Metabolomics
Background:
- Liquid chromatography-mass spectrometry (LC-MS) is a highly sensitive technique for metabolite detection.
- It accommodates a wide range of chemical properties and molecular masses.
- Various chromatographic and MS configurations exist for different analytical needs.
Purpose of the Study:
- To describe a method for detecting a broad spectrum of metabolites using LC-MS.
- To present a technique for isolating specific target metabolites.
- To detail the application of reversed-phase (C18) LC-MS in metabolite analysis.
Main Methods:
- Fractionation of biological extracts using chromatography on a suitable stationary phase.
- Direct transfer of eluent to the mass spectrometry ionization source.
- Utilizing reversed-phase (C18) liquid chromatography coupled with mass spectrometry (LC-MS).
Main Results:
- Successful detection of a wide array of metabolites.
- Demonstration of metabolite isolation capabilities.
- Establishment of a robust LC-MS method for comprehensive metabolic profiling.
Conclusions:
- Reversed-phase C18 LC-MS is effective for broad metabolite detection.
- The described methods allow for both comprehensive profiling and targeted isolation of metabolites.
- LC-MS provides a powerful tool for analyzing diverse biological samples.
