Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptomic and proteomic assessment of radiation injury and dose-rate dependency in white blood cells.

Journal of radiological protection : official journal of the Society for Radiological Protection·2026
Same author

Immunoproteomics: The Evolution from Classical Methods to Emerging Techniques.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Serological Proteome Analysis for Antigen Identification.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Editorial: The role of microbial glycosylation in host-pathogen interactions.

Frontiers in cellular and infection microbiology·2025
Same author

Dose-response modeling reveals multifaceted molecular responses to low-dose radiation in human white blood cells.

International journal of radiation biology·2025
Same author

Polar flagellin glycan heterogeneity of Aeromonas hydrophila strain ATCC 7966<sup>T</sup>.

Bioorganic chemistry·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Immunoproteomics: current technology and applications.

Kelly M Fulton1, Susan M Twine

  • 1Human Health Therapeutics, National Research Council Canada, Ottawa, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2013
PubMed
Summary
This summary is machine-generated.

Immunoproteomics identifies disease-related antigens using advanced techniques. This field helps understand immune responses, discover vaccine candidates, and find biomarkers for diseases.

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Related Experiment Videos

Last Updated: May 8, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
14:51

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

Area of Science:

  • Immunology
  • Proteomics
  • Biochemistry

Background:

  • Adaptive immune responses rely on presented peptides from pathogens or cancer cells.
  • Classical immunological assays like ELISA and Western blotting have limitations in protein identification.
  • Advances in genomics and proteomics have driven progress in identifying immune targets.

Purpose of the Study:

  • To provide an overview of immunoproteomics and related technologies.
  • To highlight the role of immunoproteomics in understanding disease and immunity.
  • To discuss methods for defining antigens targeted by the immune system.

Main Methods:

  • Gel-based approaches
  • Array-based methods
  • Mass spectrometry
  • DNA-based techniques
  • In silico analysis

Main Results:

  • Immunoproteomics enables identification and measurement of antigenic peptides and proteins.
  • This approach is crucial for understanding disease mechanisms and progression.
  • It aids in the discovery of novel vaccine candidates and biomarkers.

Conclusions:

  • Immunoproteomics offers powerful tools for dissecting the adaptive immune response.
  • It is advancing our understanding of host-pathogen interactions and cancer immunity.
  • The field is essential for developing new diagnostics and therapeutics.