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Related Experiment Video

Updated: May 8, 2026

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

Putting molecules in their place.

Bertrand P Cinquin¹, Myan Do, Gerry McDermott

¹Department of Anatomy, University of California San Francisco, San Francisco, California.

Journal of Cellular Biochemistry

|August 23, 2013

Summary

This summary is machine-generated.

Correlative imaging combines cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT) for detailed 3D cell analysis. This approach integrates molecular localization with high-resolution cell structure imaging in a near-native state.

Keywords:

CORRELATED IMAGING FLUORESCENCE MICROSCOPY SOFT X-RAY TOMOGRAPHY

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Published on: July 12, 2016

Area of Science:

Cell Biology
Microscopy
Biophysics

Background:

Individual microscopy techniques have limitations in capturing comprehensive cell structure and molecular organization.
Correlative imaging, combining data from multiple microscopes, offers a solution to overcome these limitations.
Emerging microscope technologies are advancing the field of correlative imaging.

Purpose of the Study:

To describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT).
To integrate 3D molecular localization data with high-resolution 3D cell reconstruction.
To present the current state and future outlook of correlative CFT-SXT.

Main Methods:

Imaging cells using cryogenic fluorescence tomography (CFT) for 3D molecular localization.
Imaging the same cells using soft X-ray tomography (SXT) for high-resolution 3D reconstruction.
Correlating data from both CFT and SXT modalities.
Imaging specimens in a near-native, cryopreserved state.

Main Results:

Successful integration of 3D molecular localization data (CFT) with high-resolution 3D cell reconstruction (SXT).
Demonstration of correlative cryogenic fluorescence tomography-soft X-ray tomography (CFT-SXT) for comprehensive cellular analysis.
Imaging cells in a cryopreserved state preserves native structure and molecular information.

Conclusions:

Correlative CFT-SXT provides a powerful method for detailed 3D cellular and molecular analysis.
This integrated approach overcomes the limitations of individual microscopy techniques.
The described method represents the current state of the art and offers promising future applications in cell biology research.