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Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
Published on: June 26, 2019
Nontraditional localization and retention signals localize human cytomegalovirus pUL34 to the nucleus
1Department of Biomedical Sciences and Program in Molecular and Cellular Biology, Heritage College of Osteopathic Medicine, Ohio University, Athens, Ohio, USA.
Abstract:
The human cytomegalovirus (HCMV) UL34 proteins localize to the nucleus. We identified a 204-amino-acid region, amino acids 40 to 244, as required for nuclear retention. A highly conserved 12-amino-acid section, amino acids 198 to 210, was required for nuclear localization. Although the nuclear localization and retention signals of pUL34 overlap the domain required for DNA-binding activity, the two regions are separable by point mutations. Our results presented here identify the first example of an HCMV protein with separable nuclear localization and retention signals.
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