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Updated: May 8, 2026

Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
Published on: July 5, 2024
Kinetic subpopulations detected by single-molecule spectroscopy: fundamental property of functional nucleic acids or
Sebastian L B König1, Danny Kowerko, Roland K O Sigel
1Institute of Inorganic Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zürich.
Abstract:
Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed.
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