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Peripheral inflammatory activation after hippocampus irradiation in the rat
Tünde Tőkés1, Gabriella Varga, Dénes Garab
1Institute of Surgical Research.
International Journal of Radiation Biology
|August 24, 2013
Summary
Hippocampus irradiation triggers peripheral inflammation and depletes liver ATP. L-alpha-glycerylphosphorylcholine (GPC) supplementation mitigates these effects, protecting against irradiation-induced inflammatory activation and energy depletion.
Area of Science:
- Neuroscience
- Biochemistry
- Radiology
Background:
- Hippocampus irradiation can induce systemic inflammatory responses.
- Assessing peripheral biochemical markers of inflammation is crucial following targeted brain irradiation.
- L-alpha-glycerylphosphorylcholine (GPC) is investigated for its potential protective effects.
Purpose of the Study:
- To detect biochemical signs of inflammatory activation in peripheral circulation after hippocampus irradiation in a rodent model.
- To evaluate the protective effects of GPC against irradiation-induced biochemical changes.
Main Methods:
- Sprague-Dawley rats underwent 40 Gy hippocampus irradiation, with or without GPC treatment (50 mg/kg, i.v.).
- Control group received saline treatment.
- Plasma histamine, TNF-α, IL-1β, IL-6, IL-10, and hepatic ATP levels were measured 3 hours post-irradiation.
Main Results:
- Hippocampus irradiation significantly decreased hepatic ATP levels.
- Irradiation led to increased plasma concentrations of TNF-α, IL-6, IL-10, and histamine.
- GPC treatment normalized liver ATP levels and reduced irradiation-induced cytokine and histamine release.
Conclusions:
- Targeted brain irradiation causes measurable pro- and anti-inflammatory cytokine changes in systemic circulation.
- GPC supplementation offers significant protection against irradiation-induced peripheral inflammation.
- GPC effectively prevents ATP depletion in the liver following hippocampus irradiation.
