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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
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Programmable interactions of functionalized single bioparticles in a dielectrophoresis-based microarray chip.

Melanie Abonnenc1, Nicolò Manaresi, Monica Borgatti

  • 1Silicon Biosystems, Bologna, Italy.

Analytical Chemistry
|August 24, 2013
PubMed
Summary

This study introduces the DEParray, a novel lab-on-a-chip platform using dielectrophoresis (DEP) to precisely manipulate and analyze single biological cells and their interactions in real-time.

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Microfluidics

Background:

  • Precise manipulation of single biological objects is crucial for advancing biomedicine.
  • Existing methods lack the capability for high-throughput, software-guided single-cell analysis.

Purpose of the Study:

  • To develop and validate a lab-on-a-chip platform for manipulating and analyzing individual biological objects.
  • To enable real-time assessment of cell-cell interactions and immunophenotypes.

Main Methods:

  • Utilized a dielectrophoresis (DEP) based lab-on-a-chip platform (DEParray) with a 320x320 electrode array.
  • Demonstrated software-guided manipulation, forced cell interactions, and on-chip immunophenotyping.
  • Assessed immune lysis in real-time using fluorescent dye release.

Main Results:

  • Successfully captured and moved single biological objects using DEP cages.
  • Enabled programmable interactions between target cells and microspheres or natural killer (NK) cells.
  • Achieved on-chip immunophenotypic discrimination and real-time immune lysis assessment.

Conclusions:

  • The DEParray platform facilitates rapid, arrayed, software-guided manipulation and analysis of single cells.
  • It serves as a discovery tool for novel cell-cell interactions and immunophenotypes.
  • This technology has potential applications in drug discovery and personalized medicine.