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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Sensitive and rapid detection of blaNDM-1 in clinical samples by isothermal cross-priming amplification
Yuehua Ke1, Yufei Wang, Zhoujia Wang
1Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100071, PR China.
Abstract:
A highly sensitive, specific diagnostic assay for detection of bla(NDM-1) based on cross priming amplification (CPA) was developed. The sensitivity ranged from 2.5 to 25 copies per reaction for different clinical samples. The highly and sensitive detection of bla(NDM-1) in clinical samples highlighted the potential clinical applications of CPA.
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