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Updated: May 8, 2026

Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
Published on: January 30, 2018
Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or
Sungchul Hohng1, Sanghwa Lee, Jinwoo Lee
1Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Korea. shohng@snu.ac.kr.
Abstract:
Since its first demonstration about twenty years ago, single-molecule fluorescence resonance energy transfer (FRET) has undergone remarkable technical advances. In this tutorial review, we will discuss two technical advances that increase the information content of the single-molecule FRET measurements: single-molecule multi-color FRET and single-molecule FRET combined with force or torque. Our expectations for future developments will be briefly discussed at the end.

