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Cell spreading on laminin substrate involves Con A-binding proteins.

R Moutsita1, J Botti, M A Doyennette-Moyne

  • 1INSERM U. 180, UFR Biomédicale des Saints-Péres, Paris, France.

Reproduction, Nutrition, Development
|January 1, 1990
PubMed
Summary
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Concanavalin A (Con A) hinders chick embryo fibroblast (CEF) spreading on laminin (LM) by interacting with specific cell surface proteins. These Con A-binding proteins are crucial for CEF adhesion and spreading on LM substrates.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Developmental Biology

Background:

  • Cell spreading on extracellular matrix substrates is crucial for tissue development and repair.
  • Laminin (LM) and fibronectin (FN) are key extracellular matrix components that influence cell behavior.
  • Lectins, like Concanavalin A (Con A), can bind to cell surface carbohydrates and modulate cellular functions.

Purpose of the Study:

  • To investigate the role of Concanavalin A (Con A) binding proteins in chick embryo fibroblast (8 d CEF) spreading on laminin (LM) and fibronectin (FN) substrates.
  • To determine if Con A-mediated inhibition of cell spreading is specific to certain extracellular matrix components.

Main Methods:

  • Utilizing Concanavalin A (Con A) to assess its effect on 8 d CEF adhesion and spreading.

Related Experiment Videos

  • Employing isolated Con A-binding glycoproteins to probe their specific inhibitory effects.
  • Testing cell responses on both laminin (LM) and fibronectin (FN) substrates.

    • Main Results:

    • Concanavalin A (Con A) impaired 8 d CEF spreading on laminin (LM) but not on fibronectin (FN) substrates.
    • Isolated Con A-binding proteins strongly interacted with Con A-Sepharose and were eluted by alpha-methylmannopyranoside.
    • These isolated proteins specifically inhibited 8 d CEF spreading on LM in a dose-dependent manner, but not on FN.

    Conclusions:

    • Cell surface Con A-binding proteins are implicated in 8 d CEF spreading specifically on laminin (LM) substrates.
    • The interaction of Con A-binding proteins with Con A is dependent on their carbohydrate moieties.
    • These findings highlight the distinct roles of extracellular matrix components in regulating cell adhesion and spreading.