Related Experiment Video
Updated: Apr 21, 2026
08:07
Simple Detection of Primary Cilia by Immunofluorescence
Published on: May 15, 2020
10.7K
Autophagy and regulation of cilia function and assembly
I Orhon1, N Dupont1, O Pampliega2
11] INSERM U1151-CNRS UMR 8253, Paris, France [2] Institut Necker Enfants-Malades (INEM), Paris, France [3] Université Paris Descartes, Sorbonne Paris Cité, Paris, France.
Cell Death and Differentiation
|November 1, 2014
Summary
Primary cilia and autophagy are linked, with cilia signaling initiating autophagy and autophagy regulating cilia. This crosstalk impacts cell biology, development, and diseases linked to cilia defects.
Area of Science:
- Cell Biology
- Molecular Biology
- Physiology
Background:
- Motile and primary cilia (PC) are microtubule-based cell surface structures crucial for motility and environmental signaling.
- Cilia play vital roles in cellular functions, integrating mechanical and chemical signals.
- Autophagy is a conserved cellular process for intracellular degradation.
Purpose of the Study:
- To explore the novel interplay between cilia and autophagy.
- To understand how cilia signaling influences autophagy.
- To investigate the role of autophagy in regulating ciliary protein levels and ciliogenesis.
Main Methods:
- Investigated signaling pathways from primary cilia.
- Examined the recruitment of autophagic machinery.
- Analyzed autophagosome formation triggered by ciliary signaling.
- Assessed the impact of autophagy on ciliogenesis and ciliary protein levels.
Main Results:
- Primary cilia signaling recruits the autophagic machinery, initiating autophagosome formation.
- Autophagy plays a regulatory role in ciliogenesis by controlling ciliary protein levels.
- A significant crosstalk exists between autophagy and ciliated structures.
Conclusions:
- The interaction between cilia and autophagy is a newly recognized area in cell biology.
- This crosstalk has substantial implications for development and physiology.
- Dysregulation of this interplay may contribute to human pathologies associated with ciliary defects.
Related Concept Videos
01:05Mechanism of Ciliary Motion
4.8K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
4.8K
01:05Mechanism of Ciliary Motion
2.6K
2.6K
01:22Microtubules in Signaling
1.5K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
1.5K
01:36Delivery Pathways to the Lysosome
7.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.6K
01:27Autophagy
5.0K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.0K
01:32Microtubule Associated Motor Proteins
9.9K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
9.9K