Related Experiment Video
Updated: Feb 11, 2026

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles
Published on: December 9, 2022
Comparison of three methods to assess individual skeletal maturity
Enzo Pasciuti1, Lorenzo Franchi, Tiziano Baccetti
1Private practice, Milan, Italy.
Background:
The knowledge of facial growth and development is fundamental to determine the optimal timing for different treatment procedures in the growing patient.
Aim:
To analyze the reproducibility of three methods in assessing individual skeletal maturity, and to evaluate any degree of concordance among them.
Design:
In all, 100 growing subjects were enrolled to test three methods: the hand-wrist, cervical vertebral maturation (CVM), and medial phalanges of the third finger method (MP3). Four operators determined the skeletal maturity of the subjects to evaluate the reproducibility of each method. After 30 days the operators repeated the analysis to assess the repeatability of each method. Finally, one operator examined all subjects' radiographs to detect any concordance among the three methods.
Results:
The weighted kappa values for inter-operator variability were 0.94, 0.91, and 0.90, for the WRI, CVM, and MP3 methods, respectively. The weighted kappa values for intra-operator variability were 0.92, 0.91, and 0.92, for the WRI, CVM, and MP3 methods, respectively.
Conclusion:
The three methods revealed a high degree of repeatability and reproducibility. Complete agreement among the three methods was observed in 70% of the analyzed samples. The CVM method has the advantage of not necessitating an additional radiograph. The MP3 method is a simple and practical alternative as it requires only a standard dental x-ray device.
Related Concept Videos
Impact of Individuals on Individuals
The Sense of Self: Reflected Self-Appraisal and Social Comparison
Renal Drug Clearance: Comparison Between Renal Excretion Methods
Renal clearance is often associated with the renal glomerular filtration rate (GFR), which represents the rate at which plasma is filtered through the glomeruli in the kidney. When drug reabsorption is minimal and there is no active secretion, renal clearance is closely related to the...
Maturation of Endosomes
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...
Classification of Skeletal Muscle Fibers
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
What is the Skeletal System?

