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In Vitro Antimicrobial Long-Term Evaluation of Corneal Preservation Media Against Microorganisms Using A

Diego R Hoshino Ruiz1, Davi Chen Wu, Ricardo Holzchuh

  • 1*Department of Ophthalmology, Santa Casa de São Paulo, São Paulo, Brazil; †Department of Ophthalmology, Hospital das Clínicas of University of São Paulo, São Paulo, Brazil; and ‡Department of Microbiology, Santa Casa de São Paulo, São Paulo, Brazil.

Cornea
|August 27, 2013
PubMed
Abstract

Insights

Optisol-GS demonstrated antimicrobial activity against several corneal infection microorganisms after 7 to 10 days. However, no antimicrobial effect was observed for any microbe within 72 hours in this in vitro study.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Infectious Diseases

Background:

  • Corneal infections pose a significant threat to vision.
  • Understanding the antimicrobial properties of ocular solutions is crucial for preventing and treating these infections.
  • Optisol-GS is a commonly used storage solution for ocular tissues.

Purpose of the Study:

  • To assess the in vitro long-term antimicrobial activity of Optisol-GS.
  • To evaluate its efficacy against microorganisms frequently implicated in corneal infections.
  • To determine the duration of antimicrobial effect using a closed-chamber model.

Main Methods:

  • Optisol-GS samples were intentionally contaminated with various corneal pathogens.
  • Microbial growth was monitored over extended periods using a closed-chamber system.
  • Macroscopic observation was employed to analyze microbial proliferation.

Main Results:

  • Bacterial growth of Staphylococcus aureus and Pseudomonas aeruginosa was noted up to 7 and 14 days, respectively.
  • Staphylococcus epidermidis, Streptococcus agalactiae, and Candida albicans showed growth throughout the study.
  • Streptococcus pneumoniae, Streptococcus pyogenes, and Escherichia coli exhibited growth for varying durations, up to 72 hours, 7 days, and 48 hours, respectively.

Conclusions:

  • Optisol-GS did not exhibit antimicrobial activity against any tested microorganism within 72 hours.
  • Effective antimicrobial activity against Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus pyogenes, Pseudomonas aeruginosa, and Escherichia coli was observed between 7 and 10 days.
  • The study highlights the time-dependent antimicrobial properties of Optisol-GS against specific ocular pathogens.

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