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Updated: May 8, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
Isolation of monomeric photosystem II that retains the subunit PsbS
Patrycja Haniewicz1, Daniele De Sanctis, Claudia Büchel
1International Institute of Molecular and Cell Biology, Ul. Ks. Trojdena 4, 02109, Warsaw, Poland.
Abstract:
Photosystem II has been purified from a transplastomic strain of Nicotiana tabacum according to two different protocols. Using the procedure described in Piano et al. (Photosynth Res 106:221-226, 2010) it was possible to isolate highly active PSII composed of monomers and dimers but depleted in their PsbS protein content. A "milder" procedure than the protocol reported by Fey et al. (Biochim Biophys Acta 1777:1501-1509, 2008) led to almost exclusively monomeric PSII complexes which in part still bind the PsbS protein. This finding might support a role for PSII monomers in higher plants.
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