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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Analysis of allele-specific gene expression using a target-oriented tiling microarray assay.

Huiyong Zhang1, Xing-Wang Deng, Lei Li

  • 1Department of Biology, University of Virginia, Charlottesville, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed a new microarray assay to measure allele-specific gene expression. This method quantifies unequal expression from parental chromosomes, advancing the study of gene regulation in heterozygous individuals.

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Last Updated: May 8, 2026

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Area of Science:

  • Molecular Biology
  • Computational Biology
  • Genetics

Background:

  • Recent advances in molecular and computational biology have improved methods for analyzing global gene expression.
  • It is often assumed that alleles contribute equally to the transcript pool, but evidence suggests imbalanced allelic expression is common.
  • This unequal expression occurs in heterozygous individuals beyond genomic imprinting.

Purpose of the Study:

  • To develop and describe a novel method for analyzing allele-specific gene expression.
  • To provide a high-throughput and multiplex approach for detecting and quantifying unequal allelic expression.

Main Methods:

  • Developed an oligonucleotide tiling microarray-based assay.
  • The assay targets single-nucleotide polymorphisms (SNPs) to differentiate between alleles.
  • Employs a two-step procedure for detection and quantification.

Main Results:

  • The described assay enables the analysis of allele-specific gene expression.
  • It effectively detects and quantifies unequal expression from different parental chromosomes.
  • The method is adaptable to various experimental systems.

Conclusions:

  • The oligonucleotide tiling microarray assay is a powerful tool for studying allele-specific gene expression.
  • This method facilitates the investigation of widespread imbalanced allelic expression in heterozygous individuals.
  • The assay offers a high-throughput, multiplex solution applicable to diverse research settings.