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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 8, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Detection of epigenetic alterations using tiling arrays.

Hoi-Hung Cheung¹, Owen M Rennert, Tin-Lap Lee

¹Laboratory of Clinical and Developmental Genomics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)

|August 27, 2013

Summary

Cancer cells exhibit altered epigenetic status due to neoplastic transformation. Genome-wide DNA methylation profiling using tiling arrays identified numerous epigenetic changes linked to testicular cancer progression.

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Area of Science:

Cancer Biology
Epigenetics
Genomics

Background:

Neoplastic transformation alters the epigenetic status of cancer cells.
Epigenetic modifications influence gene expression, chromatin organization, and tumor suppression.
Genome-wide mapping is crucial for identifying cancer-specific epigenetic alterations.

Purpose of the Study:

To investigate the methylome of testicular cancers using genome-wide approaches.
To identify epigenetic hotspots associated with testicular cancer development and progression.

Main Methods:

Utilized DNA tiling arrays for whole-genome methylation profiling.
Applied high-density DNA microarrays for comprehensive epigenetic mark analysis.

Main Results:

Successfully profiled the methylome of testicular cancer samples.
Identified numerous epigenetically modified loci resulting from tumor progression.

Conclusions:

Epigenetic alterations are a hallmark of testicular cancer.
DNA tiling arrays are effective tools for discovering cancer-associated epigenetic changes.