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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 8, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Inference of alternative splicing from tiling array data.

Johannes Eichner1

  • 1Center for Bioinformatics, University of Tuebingen, Tübingen, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2013
PubMed
Summary
This summary is machine-generated.

Alternative splicing (AS) regulates gene expression by creating diverse mRNA isoforms. This study explores AS in plants, revealing its tissue-specific regulation and response to environmental stresses using advanced computational methods.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Alternative splicing (AS) is a key eukaryotic gene expression mechanism, generating diverse mRNA isoforms.
  • AS contributes to cell and tissue functional diversification.
  • Environmental stresses significantly impact AS, leading to novel transcript isoforms.

Purpose of the Study:

  • To investigate genome-wide alternative splicing in plants.
  • To understand tissue-specific splicing regulation.
  • To analyze the effects of environmental conditions on pre-mRNA splicing.

Main Methods:

  • Development and application of traditional statistical and machine learning-based methods for AS detection.
  • Analysis of AS events using tiling array data.
  • Profiling of AS in the model plant *A. thaliana*.

Main Results:

  • Detection and profiling of alternative splicing events across various tissues and stress conditions.
  • Identification of novel transcript isoforms in response to biotic and abiotic stresses.
  • Insights into genome-wide AS regulation in plants.

Conclusions:

  • Large-scale AS monitoring is crucial for understanding plant gene expression.
  • AS plays a significant role in plant adaptation to environmental changes.
  • Computational approaches are effective for AS detection and profiling.