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Updated: May 8, 2026
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
Published on: November 15, 2013
A nonradioactive electrophoretic mobility shift assay for measurement of pregnane X receptor binding activity to
Aneta Vavrova1, Radim Vrzal, Zdenek Dvorak
1Department of Cell Biology and Genetic, Faculty of Science, Palacky University Olomouc, Slechtitelu, Olomouc, Czech Republic.
Abstract:
The electrophoretic mobility shift assay (EMSA) is a method for the study of specific DNA–protein interactions in vitro. The pregnane X receptor (PRX) is a key xenobiotic sensor that regulates the expression of drug-metabolizing enzymes andmany other genes. Radiolabeled ³²P-DNA-probes had been used in studies of PXR-DNA interactions. There is an increasing need for nonradioactive assays, due to the health, safety and environmental issues. In the current study, we present a protocol for the nonradioactive electrophoretic mobility shift assay, allowing studying interactions between human PXR with promoter DNA sequences.
