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Published on: June 14, 2024
The first transformation method for the thermo-acidophilic archaeon Thermoplasma acidophilum
Erzsébet Baka1, Sándor Varga, Cédric Hobel
1Department of Environmental Protection & Environmental Safety, Szent István University, Gödöllő 2100, Hungary; Department of Microbiology, Central Environmental and Food Science Research Institute, Budapest 1022, Hungary.
Abstract:
A transformation method yielding up to 10(4) transformants per μg circular DNA was developed for Thermoplasma acidophilum. The method is based on a natural DNA uptake process in which T. acidophilum cells keep their integrity and turn competent at pH 3.5 and 58°C. Shuttle vector maintenance could not be detected, since the used Nov(R) gyraseB gene integrated into its chromosomal counterpart by homologous recombination.
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