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Related Concept Videos

The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
DNA Replication02:40

DNA Replication

DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication uses a large number of...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Related Experiment Video

Updated: May 8, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

Spatiotemporal visualization of DNA replication dynamics.

Marius Reinhart1, Corella S Casas-Delucchi, M Cristina Cardoso

  • 1Department of Biology, Technische Universität Darmstadt, Darmstadt, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 28, 2013
PubMed
Summary

This study details methods for visualizing DNA replication in cells. Researchers can now track DNA copying in living cells with high spatial and temporal resolution using advanced imaging techniques.

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Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

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Related Experiment Videos

Last Updated: May 8, 2026

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
07:18

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Published on: October 27, 2011

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA replication is essential for transmitting genetic information and cellular development.
  • Previous understanding of DNA replication was based on in vitro biochemical and yeast genetic analyses.
  • Recent technological advancements enable in vivo studies of DNA replication within intact cells.

Purpose of the Study:

  • To describe detailed methodologies for studying DNA replication in vivo.
  • To enable high spatial resolution imaging of DNA replication processes.
  • To facilitate high temporal resolution imaging of DNA replication in living cells.

Main Methods:

  • Multiple DNA replication labeling and detection techniques for high spatial resolution.
  • Live-cell microscopy for tracking DNA replication dynamics over time.
  • In vivo imaging within the context of intact cells.

Main Results:

  • Established protocols for high-resolution spatial imaging of DNA replication.
  • Developed methods for real-time temporal monitoring of DNA replication in living cells.
  • Enabled detailed dissection of DNA replication mechanisms in a cellular context.

Conclusions:

  • Advanced imaging techniques provide unprecedented insights into DNA replication.
  • In vivo studies offer a more comprehensive understanding of this fundamental biological process.
  • The described methods facilitate further research into DNA replication fidelity and regulation.