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Updated: May 8, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Published on: January 31, 2018
The dynamics of DNA damage repair and transcription
Niraj M Shanbhag1, Roger A Greenberg
1Department of Cancer Biology, Abramson Family Cancer Research Institute, Basser Research Center for BRCA1/2, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Abstract:
Recent advances have led to several systems to study transcription from defined loci in living cells. It has now become possible to address long-standing questions regarding the interplay between the processes of DNA damage repair and transcription-two disparate processes that can occur on the same stretch of chromatin and which both lead to extensive chromatin change. Here we describe the development of a system to create enzymatically induced DNA double-strand breaks (DSBs) at a site of inducible transcription and methods to study the interplay between these processes.
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