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Nanohydroxyapatite incorporated electrospun polycaprolactone/polycaprolactone-polyethyleneglycol-polycaprolactone
K R Remya1, Jasmin Joseph, Susan Mani
1Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram 695012, India.
Journal of Biomedical Nanotechnology
|August 29, 2013
Summary
Biodegradable fibrous scaffolds made from polycaprolactone (PCL) and its blends show promise for tissue engineering. The PCL/CEC/nHAP scaffold demonstrated superior cell viability and proliferation, indicating potential for bony construct fabrication.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Polymer Science
Background:
- Biodegradable fibrous scaffolds are crucial for tissue regeneration.
- Polycaprolactone (PCL) is a widely used biomaterial, but its properties can be enhanced.
- Incorporating copolymers and nanoparticles can improve scaffold performance.
Purpose of the Study:
- To comparatively evaluate the physical and biological properties of electrospun PCL-based scaffolds.
- To investigate the influence of a polycaprolactone-polyethyleneglycol-polycaprolactone (CEC) copolymer and nanohydroxyapatite (nHAP) particles on scaffold characteristics.
- To assess the potential of these scaffolds for bone tissue engineering applications.
Main Methods:
- Electrospinning of polycaprolactone (PCL) and its blends with CEC and nHAP.
- Characterization of fiber morphology, porosity, wettability, and mechanical properties.
- In vitro degradation studies in phosphate-buffered saline (PBS).
- Cytotoxicity assessment using MTT assay with L929 cells.
- Evaluation of cell viability, proliferation, and osteogenic differentiation (ALP activity) using specific assays.
Main Results:
- The presence of CEC and nHAP significantly influenced the physical properties of electrospun PCL scaffolds.
- Scaffold degradation in PBS affected morphology and mechanical strength, with tensile strength decreasing significantly over 90 days.
- All tested scaffolds were non-cytotoxic, with the PCL/CEC/nHAP scaffold showing enhanced cell viability and proliferation.
- Osteogenic-induced Rabbit adipose-derived mesenchymal stem cells (ADMSCs) were confirmed on all scaffolds, indicated by ALP activity.
Conclusions:
- The PCL/CEC/nHAP scaffold exhibits promising cytocompatibility and enhanced biological performance.
- These findings highlight the potential of the PCL/CEC/nHAP scaffold for fabricating living bony constructs.
- The study provides valuable insights into designing advanced biomaterials for bone tissue engineering.
