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Measuring Single-Cell Aging with an Imaging-based Biomarker of Chromatin and Epigenetic Aging
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Thermal modifications of root transparency and implications for aging: a pilot study.

Daniele Gibelli1, Danilo De Angelis, Francesca Rossetti

  • 1LABANOF, Laboratorio di Antropologia e Odontologia Forense, Sezione di Medicina Legale, DMU - Dipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy.

Journal of Forensic Sciences
|August 29, 2013
PubMed
Summary

Root transparency, used for age estimation, is significantly altered by heat. This study found that increasing temperatures drastically reduce root transparency, impacting its reliability for forensic age assessment.

Keywords:
Lamendinage estimationforensic anthropologyforensic odontologyforensic sciencesheatingroot transparency

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Area of Science:

  • Forensic Anthropology
  • Odontology
  • Bioarchaeology

Background:

  • Root transparency in teeth is a recognized indicator for dental age estimation.
  • The effect of heat on root transparency is largely unknown, limiting its use in evaluating burnt human remains.

Purpose of the Study:

  • To investigate the impact of controlled heat exposure on tooth root transparency.
  • To assess the reliability of root transparency for age estimation in thermally altered dental samples.

Main Methods:

  • A pilot study involving 105 human teeth from dental patients and autopsy.
  • Teeth were subjected to controlled heating at 50°C, 100°C, 150°C, and 200°C.
  • Root transparency was measured before and after heat exposure to quantify changes.

Main Results:

  • A significant, temperature-dependent decrease in root transparency was observed.
  • At 200°C, 77% of samples showed reduced root transparency.
  • A strong correlation (CI = 0.96) was found between increased temperature and decreased transparency.

Conclusions:

  • Thermal alteration radically modifies tooth root transparency.
  • Caution is advised when applying root transparency methods for age estimation to burnt remains.
  • Further research is needed to understand the precise mechanisms and extent of heat-induced changes.