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Related Concept Videos

DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...

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Related Experiment Video

Updated: May 8, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

A core hSSB1-INTS complex participates in the DNA damage response.

Feng Zhang1, Teng Ma, Xiaochun Yu

  • 1Division of Molecular Medicine and Genetics, Department of Internal Medicine, University of Michigan Medical School, 1150 W. Medical Center Drive, 5560 MSRBII, Ann Arbor, MI 48109, USA.

Journal of Cell Science
|August 30, 2013
PubMed
Summary

Human single-stranded DNA-binding protein 1 (hSSB1) and integrator complex subunit 6 (INTS6) form a complex crucial for DNA repair. This complex aids in genomic stability by regulating DNA damage site accumulation of key proteins.

Keywords:
DNA damage responseHomologous recombinationhSSB1–INTS complex

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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
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Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

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Related Experiment Videos

Last Updated: May 8, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Human single-stranded DNA-binding protein 1 (hSSB1) is vital for DNA damage response and genomic stability.
  • The core hSSB1 complex previously included hSSB1, INTS3, and C9orf80.

Purpose of the Study:

  • To identify novel subunits of the core hSSB1 complex.
  • To elucidate the role of new subunits in the DNA damage response pathway.

Main Methods:

  • Protein affinity purification was employed to identify interacting proteins.
  • In vitro and in vivo experiments confirmed complex formation and interactions.
  • Localization studies tracked protein relocation to DNA damage sites.

Main Results:

  • Integrator complex subunit 6 (INTS6) was identified as a major subunit of the core hSSB1 complex.
  • INTS6 forms a stable complex with INTS3 and hSSB1, with direct interaction between INTS6 and INTS3.
  • The hSSB1-INTS complex, including INTS6, relocates to DNA damage sites during the DNA damage response.
  • This complex regulates the accumulation of RAD51 and BRCA1 at damage sites, impacting homologous recombination.

Conclusions:

  • INTS6 is a critical component of the core hSSB1 complex involved in DNA repair.
  • The hSSB1-INTS complex plays a regulatory role in homologous recombination by influencing RAD51 and BRCA1 recruitment.