Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
Synthesis of Phosphatidylcholine in the ER Membrane01:27

Synthesis of Phosphatidylcholine in the ER Membrane

The ER synthesizes lipids for building cell membranes and performing cellular functions such as energy storage and signaling. The lipid synthesis machinery embedded in the ER membrane primarily collects all reactants from the cytosol. Following synthesis, the secretory pathway and the ER contact sites distribute these lipids to other cellular organelles. Additionally, the energy-rich triacylglycerides are transported from the ER via lipid droplets.
The major components of all eukaryotic cell...
Biosynthesis of Lipids01:29

Biosynthesis of Lipids

Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis pathway, which...
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Cells of the Epidermis01:24

Cells of the Epidermis

The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Wnt-associated DKK3 in keratinocytes mediates radiation-induced hyperplasia, dermatitis and skin fibrosis.

Signal transduction and targeted therapy·2026
Same author

Predicting GD2 expression across cancer types by the integration of pathway topology and transcriptome data.

Frontiers in bioinformatics·2025
Same author

The SGLT2 Inhibitor Dapagliflozin Disrupts the Cell Cycle at High Concentrations Without Altering Glycosphingolipid (De Novo)Biosynthesis.

International journal of molecular sciences·2025
Same author

Ganglioside Profiling Uncovers Distinct Patterns in High-Risk Neuroblastoma.

International journal of molecular sciences·2025
Same author

Glucosylceramide Synthase Inhibition in Combination with Aripiprazole Sensitizes Hepatocellular Cancer Cells to Sorafenib and Doxorubicin.

International journal of molecular sciences·2025
Same author

Unraveling the glycosphingolipid metabolism by leveraging transcriptome-weighted network analysis on neuroblastic tumors.

Cancer & metabolism·2024
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Related Experiment Video

Updated: May 8, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Ceramide synthesis in the epidermis.

Mariona Rabionet1, Karin Gorgas2, Roger Sandhoff3

  • 1Lipid Pathobiochemistry Group within Department of Cellular & Molecular Pathology, German Cancer Research Center (dkfz.), Heidelberg, Germany.

Biochimica Et Biophysica Acta
|August 31, 2013
PubMed
Summary
This summary is machine-generated.

Skin barrier integrity relies on ceramides, essential lipids forming the stratum corneum's lamellar matrix. This review explores epidermal ceramide diversity, biosynthesis, and regulation for maintaining skin's protective function.

Keywords:
1-O-AcylceramidesAutosomal recessive congenital ichthyosis (ARCI)Ceramide synthesisEpidermisOmega-esterified ceramidesSkin barrier function

More Related Videos

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Related Experiment Videos

Last Updated: May 8, 2026

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
08:49

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Published on: May 28, 2021

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
09:32

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Published on: September 26, 2019

Area of Science:

  • Dermatology
  • Biochemistry
  • Skin Biology

Background:

  • The epidermis, particularly the stratum corneum, acts as a critical barrier against water loss, pathogens, and xenobiotics.
  • A lipid-enriched lamellar matrix embedding corneocytes is vital for this epidermal permeability barrier.
  • Ceramides are key components of these intercellular structures, with specific types crucial for skin barrier integrity.

Purpose of the Study:

  • To review the diversity of epidermal ceramides, including 1-O-acylceramides.
  • To focus on epidermal ceramide biosynthesis, its metabolic and topological needs, and potential hydroxylating enzymes.
  • To discuss the regulation of epidermal ceramides, including transcription factors and liposensors involved in skin lipid metabolism and barrier homeostasis.

Main Methods:

  • Literature review focusing on epidermal ceramides.
  • Analysis of ceramide biosynthesis pathways and regulatory mechanisms.
  • Discussion of enzymes involved in ceramide hydroxylation.

Main Results:

  • Epidermal ceramides exhibit significant diversity, with linoleic acid- and protein-esterified ceramides being critical for barrier structure.
  • Key enzymes potentially involved in α- and ω-hydroxylation of ceramides are identified.
  • Transcription factors and liposensors play significant roles in regulating skin lipid metabolism and epidermal barrier homeostasis.

Conclusions:

  • Understanding epidermal ceramide diversity, biosynthesis, and regulation is crucial for maintaining skin barrier function.
  • Specific ceramide structures and their metabolic pathways are vital for skin's protective capabilities.
  • Further research into ceramide metabolism and regulation can inform strategies for managing skin barrier disorders.