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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

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Related Experiment Video

Updated: May 8, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

DIELECTROPHORESIS BASED MICRO FLOW CYTOMETRY.

Jody Vykoukal, Jon A Schwartz, Peter R C Gascoyne

Micro Total Analysis Systems : Proceedings of the ... [Mu] TAS International Conference on Miniaturized Chemical and Biochemical Analysis Systems. [Mu] TAS (Conference)

|August 31, 2013

Summary

This summary is machine-generated.

A simplified flow cytometer uses negative dielectrophoresis for particle focusing. This inexpensive, compact system offers robust cell and particle characterization for various applications.

Keywords:

cell analysis dielectrophoresis flow cytometry particle focusing

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Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

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Last Updated: May 8, 2026

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Published on: August 7, 2014

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

Area of Science:

Biomedical Engineering
Analytical Chemistry
Microfluidics

Background:

Traditional flow cytometers can be expensive and complex.
Need for accessible and robust cell and particle analysis tools.

Purpose of the Study:

To develop a simplified, cost-effective, and compact flow cytometer.
To enable robust cell and particle characterization using integrated detection methods.

Main Methods:

Utilized negative dielectrophoresis (DEP) for precise particle focusing.
Integrated optical and AC impedance detectors for simultaneous detection.
Designed a modular system for flexibility and ease of use.

Main Results:

Achieved an inexpensive, compact, and robust system for particle analysis.
Demonstrated the effectiveness of DEP for particle focusing in the system.
Validated the integrated optical and AC impedance detection for characterization.

Conclusions:

The simplified flow cytometer design offers a viable alternative for cell and particle characterization.
The modular design allows for standalone use or integration into micro total analysis systems.
This technology can increase accessibility to flow cytometry for research and diagnostics.