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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
Published on: November 14, 2025
863
High-Throughput Microfluidic Device for Circulating Tumor Cell Isolation from Whole Blood
Daniel K Yang1, Serena Leong2, Lydia L Sohn1
1Dept. of Mechanical Engineering, University of California, Berkeley, CA 94720 USA.
Micro Total Analysis Systems : Proceedings of the ... [Mu] TAS International Conference on Miniaturized Chemical and Biochemical Analysis Systems. [Mu] TAS (Conference)
|July 26, 2016
Summary
We developed a microfluidic device to isolate circulating tumor cells (CTCs) from blood. This technology shows high recovery rates, aiding cancer patient prognosis and therapy tracking.
Area of Science:
- Biomedical Engineering
- Oncology
- Microfluidics
Background:
- Circulating tumor cells (CTCs) are crucial biomarkers for cancer prognosis and monitoring treatment efficacy.
- Accurate isolation of CTCs from whole blood is essential for clinical applications.
Purpose of the Study:
- To develop and validate a novel microfluidic device for efficient CTC isolation.
- To assess the device's performance in recovering cancer cells from whole blood.
Main Methods:
- A multi-stage microfluidic device was designed utilizing inertial and Dean drag forces.
- The device was tested using whole blood samples spiked with MCF-7 GFP cancer cells.
Main Results:
- The microfluidic device achieved a high recovery rate of 94.2% ± 2.1% for cancer cells.
- Demonstrated effective isolation of CTCs from complex blood matrices.
Conclusions:
- The developed microfluidic device offers a promising tool for CTC isolation.
- This technology can enhance the clinical utility of CTCs for cancer patient management.
