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Isolation of Murine Lymph Node Stromal Cells

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Published on: August 19, 2014

Extrathymic Aire-expressing cells are a distinct bone marrow-derived population that induce functional inactivation

James M Gardner¹, Todd C Metzger, Eileen J McMahon

¹Diabetes Center, University of California, San Francisco, San Francisco, CA 94143-0540, USA; Department of Surgery, University of California, San Francisco, San Francisco, CA 94143-0540, USA.

|September 3, 2013

Abstract:

The autoimmune regulator (Aire) is essential for prevention of autoimmunity; its role is best understood in the thymus, where it promotes self-tolerance through tissue-specific antigen (TSA) expression. Recently, extrathymic Aire-expressing cells (eTACs) have been described in murine secondary lymphoid organs, but the identity of such cells and their role in immune tolerance remains unclear. Here we have shown that eTACs are a discrete major histocompatibility complex class II (MHC II)(hi), CD80(lo), CD86(lo), epithelial cell adhesion molecule (EpCAM)(hi), CD45(lo) bone marrow-derived peripheral antigen-presenting cell (APC) population. We also have demonstrated that eTACs can functionally inactivate CD4⁺ T cells through a mechanism that does not require regulatory T cells (Treg) and is resistant to innate inflammatory stimuli. Together, these findings further define eTACs as a distinct tolerogenic cell population in secondary lymphoid organs.

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