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Related Concept Videos

DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the lytic replication...
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...

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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
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Published on: February 16, 2014

Recombinant bacteriophages as gold binding bio-templates.

Nuriye Korkmaz1

  • 1Convergence Bioscience Group, Nanomedicine Team, Korea Institute of Science and Technology, Europe Forschungsgesellschaft mbH, Campus E 71, D-66123 Saarbrücken, Germany.

Colloids and Surfaces. B, Biointerfaces
|September 3, 2013
PubMed
Summary

Genetically engineered filamentous bacteriophages exhibit enhanced metal binding. This modification facilitates their interaction with gold surfaces and nanoparticles, paving the way for novel bio-applications.

Keywords:
BacteriophageGenetic engineeringGoldMetallizationNanoparticleSEM

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Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

Area of Science:

  • Biotechnology
  • Materials Science
  • Nanotechnology

Background:

  • Bacteriophages are viruses that infect bacteria.
  • Filamentous bacteriophages, like fd-bacteriophages, have potential applications in nanotechnology.
  • Genetic engineering can modify bacteriophage properties.

Purpose of the Study:

  • To enhance the metal binding capabilities of filamentous fd-bacteriophages through genetic engineering.
  • To investigate the interaction of engineered bacteriophages with gold surfaces and nanoparticles.
  • To explore the potential of these engineered phages in bio-templated nanomaterial synthesis.

Main Methods:

  • Genetic engineering of fd-bacteriophage major coat protein (p8) to express a metal-binding amino acid sequence (MMM).
  • Quartz crystal microbalance (QCM) for analyzing binding kinetics.
  • UV-vis absorption spectroscopy and Scanning Electron Microscopy (SEM) for characterization.
  • Electroless deposition of gold nanoparticles and Energy Dispersive X-ray Spectroscopy (EDX) for elemental analysis.

Main Results:

  • Expression of the MMM sequence on phage p8 significantly enhanced binding to gold surfaces and gold nanoparticles (AuNPs) via gold-sulfur (AuS) interactions.
  • Successful deposition of gold particles onto phage assemblies through chemical reduction.
  • EDX confirmed the presence of gold on both AuNP-decorated and chemically metallized phage structures.

Conclusions:

  • Genetic modification of bacteriophages can impart specific metal-binding properties.
  • Engineered bacteriophages can serve as scaffolds for gold nanoparticle assembly.
  • These findings support the development of bio-templated nanowires and biosensors.