Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lytic Cycle of Bacteriophages01:30

Lytic Cycle of Bacteriophages

70.8K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.8K
Lysogenic Cycle of Bacteriophages00:43

Lysogenic Cycle of Bacteriophages

62.2K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GLP-1 Receptor Agonists for Weight Loss and Risk of Major Safety Outcomes: A Multicentre Cohort Study.

Diabetes, obesity & metabolism·2026
Same author

Risk of arrhythmia following ankylosing spondylitis, 2012-2023: a nationwide cohort study.

Clinical rheumatology·2026
Same author

Differential roles of DTI and EEG in predicting cognitive function after left basal ganglia stroke: a proof-of-concept study.

Scientific reports·2026
Same author

Surface-Functionalized LLZO-Incorporated Multilayer Composite Solid Electrolytes for Dendrite Suppression and Efficient Ionic Conduction in Lithium-Metal Batteries.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Electroreduction of carbon dioxide (CO<sub>2</sub>) at oxalate and polypyrrole modified copper surfaces.

Nanoscale·2026
Same author

Design Guidelines for Anode Modifications to Eliminate Soft Short Circuits in Anode-Free Na Batteries Under High Current Density With Large-Capacity Plating, Demonstrated via Initiated Chemical Vapor Deposition (iCVD).

Angewandte Chemie (International ed. in English)·2026
Same journal

AI-Derived Smart Microneedle Systems for Advanced Wound Management: From Intelligent Sensing to Closed-Loop Therapy.

Macromolecular bioscience·2026
Same journal

A Novel Chitosan-Gelatin Scaffold and Cell Spray Therapy for Treating Limbal Stem Cell Deficiency.

Macromolecular bioscience·2026
Same journal

Electroconductive Soft Microcarriers for Suspension Culture of Skeletal Muscle Cells.

Macromolecular bioscience·2026
Same journal

Dual-Responsive Chitosan-Grafted PNIPAAm Hydrogel Eye Drop Incorporating Insulin-Imprinted Microgels for Dry Eye Syndrome Treatment.

Macromolecular bioscience·2026
Same journal

Levan Inspired Hybrid Composites Materials: Bridging Natural Polysaccharides with Biomedical Technology.

Macromolecular bioscience·2026
Same journal

Anion-Specific Mechanisms in Fibrinogen Self-Assembly: Contrasting Effects of Phosphates and Chlorides in Nanofiber Formation.

Macromolecular bioscience·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

944

Bacteriophage Engineering for Improved Copper Ion Binding.

Nuriye Korkmaz1, Sandiego Himawan1,2, Muhammed Usman1

  • 1Biosensor Group, Korea Institute of Science and Technology Europe Forschungsgesellschaft mbH, Campus E 7.1, D-66123, Saarbrücken, Germany.

Macromolecular Bioscience
|November 20, 2023
PubMed
Summary
This summary is machine-generated.

Genetically engineered viruses selectively bind copper (II) ions, demonstrating potential for bioremediation. These modified fd viruses offer a novel biomaterial for environmental metal cleanup.

Keywords:
bioremediationcopperfd phagesgenetic engineeringphage display

More Related Videos

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.5K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.1K

Related Experiment Videos

Last Updated: Jul 10, 2025

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides
07:19

Author Spotlight: Efficiently Eliminating Bacteriophages from Infected Salmonella Cultures Using Lipopolysaccharides

Published on: June 28, 2024

944
Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores
11:38

Quantifying the Binding Interactions Between CuII and Peptide Residues in the Presence and Absence of Chromophores

Published on: April 5, 2022

2.5K
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
12:36

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Published on: February 16, 2014

34.1K

Area of Science:

  • Biomaterials Science
  • Environmental Biotechnology
  • Molecular Biology

Background:

  • Filamentous fd phages are versatile platforms for genetic modification.
  • Peptide sequences can be engineered onto phage surfaces for specific metal ion binding.
  • Bioremediation strategies are crucial for removing toxic metal species from the environment.

Purpose of the Study:

  • To genetically engineer fd viruses to display peptide variants for selective copper (II) ion binding.
  • To characterize the physical and chemical properties of engineered viruses.
  • To evaluate the efficacy of engineered viruses in copper (II) ion sorption.

Main Methods:

  • Genetic modification of fd viruses to display peptide variants (H, HG, HGF, HGFA, HGFAN, HGFANV, HGFANVA).
  • Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM) for structural analysis.
  • Energy Dispersive X-ray Spectroscopy (EDX), Enzyme-Linked Immunosorbent Assay (ELISA), Agarose Gel Electrophoresis (AGE), Zeta Potential, and Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for characterization and binding studies.

Main Results:

  • Engineered phages maintained typical filamentous structures.
  • HGFANVA viruses formed larger assemblies and showed selective Cu(II) binding after treatment.
  • EDX confirmed the presence of organic material and Cu(II) in engineered viruses.
  • ELISA and ICP-MS demonstrated enhanced Cu(II) binding by engineered viruses compared to wild-type.
  • AGE and zeta potential analyses indicated negative surface charges.

Conclusions:

  • Genetically engineered fd viruses effectively bind Cu(II) ions.
  • These virus-based biomaterials show promise for bioremediation of metal contaminants.
  • The study presents a bottom-up approach for constructing functional biomaterials from engineered viruses.