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Related Experiment Video

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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
13:51

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Single-nanocrystal sensitivity achieved by enhanced upconversion luminescence.

Jiangbo Zhao1, Dayong Jin, Erik P Schartner

  • 1Advanced Cytometry Laboratories, MQ Photonics Research Centre and MQ BioFocus Research Centre, Macquarie University, Sydney, New South Wales 2109, Australia.

Nature Nanotechnology
|September 3, 2013
PubMed
Summary

High excitation irradiance boosts upconversion nanocrystal brightness by enabling higher activator ion concentrations, overcoming luminescence quenching. This breakthrough enhances sensitivity for applications like biodetection and bioimaging.

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Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation

Published on: August 30, 2017

Area of Science:

  • Materials Science
  • Nanotechnology
  • Photonics

Background:

  • Upconversion nanocrystals (UCNs) convert infrared light to visible light, enabling applications in biodetection, bioimaging, solar cells, and displays.
  • Current UCNs suffer from limited emission brightness due to luminescence quenching at higher activator ion doping concentrations.

Purpose of the Study:

  • To overcome the limitations of concentration quenching in UCNs.
  • To enhance the luminescence intensity of UCNs by increasing activator ion concentration.
  • To demonstrate a novel method for sensitive remote tracking using bright UCNs.

Main Methods:

  • Investigated the effect of high excitation irradiance on upconversion luminescence.
  • Increased the concentration of thulium ions (Tm(3+)) in sodium yttrium fluoride (NaYF₄) nanocrystals from 0.5 mol% to 8 mol%.
  • Utilized a microstructured optical-fibre dip sensor for remote tracking of single nanocrystals.

Main Results:

  • High excitation irradiance effectively alleviated concentration quenching, allowing for higher activator ion doping.
  • Luminescence signals were significantly enhanced, by up to a factor of 70, with increased Tm(3+) concentration.
  • Demonstrated remote tracking of a single UCN with a three-orders-of-magnitude improvement in sensitivity compared to quantum dots.

Conclusions:

  • High excitation irradiance is a viable strategy to enhance UCN brightness by enabling higher doping concentrations.
  • The developed bright UCNs offer unprecedented sensitivity for remote detection applications.
  • This work paves the way for more sensitive biodetection and bioimaging tools.