High throughput detection of antibody self-interaction by bio-layer interferometry.

Area of Science:

• Biotechnology
• Protein Engineering
• Pharmaceutical Development

Background:

• Antibody self-interaction can cause aggregation, reduced solubility, and increased viscosity, complicating drug development.
• Existing methods like self-interaction chromatography (SIC) and cross-interaction chromatography (CIC) aid in identifying problematic antibodies but can be time-consuming.

Purpose of the Study:

• To introduce a high-throughput method for detecting antibody clone self-interaction (CSI).
• To enable rapid screening of numerous antibody candidates for developability early in the discovery process.

Main Methods:

• Development and application of a novel antibody clone self-interaction assay using bio-layer interferometry (BLI) technology.
• Correlation of CSI-BLI results with established chromatography techniques (SIC and CIC).

Main Results:

• The CSI-BLI assay successfully detected antibody self-interaction.
• Antibodies exhibiting strong self-interaction in CSI-BLI also demonstrated delayed retention times in SIC and CIC.
• The method facilitated the screening of hundreds of candidates within hours, consuming minimal material.

Conclusions:

• The CSI-BLI assay is a rapid, high-throughput tool for assessing antibody developability.
• This method accelerates the identification of promising antibody leads by efficiently detecting self-interaction properties.
• This technology offers a significant advancement over traditional methods for early-stage antibody screening.