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Updated: May 8, 2026
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Published on: April 1, 2017
Quantification of protein complexes by blue native electrophoresis
Juliana Heidler1, Valentina Strecker, Florian Csintalan
1Department of Molecular Hematology, University of Frankfurt Medical School, Frankfurt, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 3, 2013
Summary
Blue native electrophoresis (BNE) analyzes native protein complexes for diagnostics and research. This study details quantification methods to complement BNE analysis of protein complexes.
Area of Science:
- Biochemistry
- Proteomics
- Molecular Biology
Background:
- Blue native electrophoresis (BNE) is a well-established technique for analyzing native protein complexes.
- BNE applications include studying protein complex composition, stoichiometry, assembly, and complexome profiling.
- It is crucial for diagnosing mitochondrial disorders and analyzing model organisms.
Purpose of the Study:
- To describe various quantification methods applicable after Blue native electrophoresis (BNE).
- To enhance the utility of BNE in functional proteomic studies requiring protein complex quantification.
Main Methods:
- The study focuses on methods for quantifying protein complexes separated by BNE.
- Specific quantification techniques are discussed in the context of BNE workflows.
Main Results:
- The abstract does not contain specific results.
- The study provides a description of quantification methods.
Conclusions:
- Quantification methods are essential for advancing functional proteomic studies using BNE.
- Integrating quantification with BNE expands its diagnostic and research capabilities.
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